発酵微生物部門の研究業績

研究論文(著書,その他)

2022年度

(研究論文)

Kataoka N,  Naoki K, Ano Y, Matsushita K, Yakushi T. Development of efficient 5-ketogluconate production system by Gluconobacter japonicusAppl Microbiol Biotechnol. 106: 7751-7761. (2022 Dec) Springer

Kataoka N, Matsutani M, Matsushita K, Yakushi T. Stepwise metabolic engineering of Corynebacterium glutamicum for the production of phenylalanine. J Gen Appl Microbiol. 2022 Aug 22. (オンライン)

Usui M, Kubota H, Ishihara M, Matsuki H, Kawabe S, Sugiura Y, Kataoka N, Matsushita K, Ano Y, Akakabe Y, Hours RA, Yakushi T, Adachi O. Histamine elimination by a coupling reaction of fungal amine oxidase and bacterial aldehyde oxidase. Biosci Biotechnol Biochem. 86: 1438-1447. (2022 Sep) 日本農芸化学会

Adachi O, Kataoka N, Matsushita K, Akakabe Y, Harada T, Yakushi T. Membrane-bound D-mannose isomerase of acetic acid bacteria: finding, characterization, and application. Biosci Biotechnol Biochem. 86 938–948 (2022 Jun) 日本農芸化学会

Nagaki K, Kataoka N, Theeragool G, Matsutani M, Ano Y, Matsushita K, Yakushi T. Periplasmic dehydroshikimate dehydratase combined with quinate oxidation in Gluconobacter oxydans for protocatechuate production. Biosci Biotechnol Biochem. 86:1151-1159. (2022 Jul) 日本農芸化学会

Kataoka N, Matsutani M, Matsumoto N, Oda M, Mizumachi Y, Ito K, Tanaka S, Kanesaki Y, Yakushi T, Matsushita K. Mutations in degP and spoT Genes Mediate Response to Fermentation Stress in Thermally Adapted Strains of Acetic Acid Bacterium Komagataeibacter medellinensis NBRC 3288. Front Microbiol. 13:802010. (2022 May)

Kataoka N, Saichana N, Matsutani M, Toyama H, Matsushita K, Yakushi  T.  Characterization of 3 phylogenetically distinct membrane-bound d-gluconate dehydrogenases of Gluconobacter spp. and their biotechnological application for efficient 2-keto-d-gluconate production. Biosci Biotechnol Biochem. 86(5):681-690. (2022 Apr) 日本農芸化学会

Haruthairat Kitwetcharoen, Ly Tu Phung, Preekamol Klanrit, Sudarat Thanonkeo, Patcharaporn Tippayawat, Mamoru Yamada and Pornthap Thanonkeo Kombucha healthy drink-recent advances in production, chemical composition and health benefits. Fermentation 9, 48 2023 MDPI

Huynh Xuan Phong, Preekamol Klanrit, Ngo Thi Phuong Dung, Sudarat Thanonkeo, Mamoru Yamada and Pornthap Thanonkeo. High-temperature ethanol fermentation from pineapple waste hydrolysate and gene expression analysis of thermotolerant yeast Saccharomyces cerevisiae. Scientific Reports 12:13965 2022 Springer Nature

Chansom Keo-oudone, Koudkeo Phommachan, Orathai Suliya, Mochamad Nurcholis, Somchanh Bounphanmy, Tomoyuki Kosaka and Mamoru YamadaHighly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii. BMC Microbiology 22:164 2022. Springer Nature

Masayuki Murata, Sornsiri Pattanakittivorakul, Toshiro Manabe, Savitree Limtong and Mamoru YamadaMutants with enhanced cellobiose-fermenting ability from thermotolerant Kluyveromyces marxianus DMKU 3-1042, which are beneficial for fermentation with cellulosic biomass. Fuels 3, 232-244. 2022 MDPI

Sornsiri Pattanakittivorakul, Tatsuya Tsuzuno, Tomoyuki Kosaka, Masayuki Murata, Yu Kanesaki, Hirofumi Yoshikawa, Savitree Limtong and Mamoru Yamada. Evolutionary adaptation by repetitive long-term cultivation with gradual increase of temperature for acquiring multi-stress tolerance and high ethanol productivity in Kluyveromyces marxianus DMKU 3-1042. Microorganisms 10, 798. 2022 MDPI

Yushi Takehara, Isamu Fijikawa, Akihiro Watanabe, Ayumi Yonemura, Tomoyuki Kosaka, Kosei Sakane, Kiyoshi Imada, Kazunori Sasaki, Hiroshi Kajihara, Shoji Sakai, Yoichi Mizukami, Muhammad Salman Haider, Sudisha Jogaiah, Shin-ichi Ito. Molecular Analysis of MgO Nanoparticle-Induced Immunity against Fusarium Wilt in Tomato. International Journal of Molecular Sciences 24(3) 2941-2941 (2023年2月2日)MDPI

赤田倫治,森寿,星田尚司,吉田知之. αシヌクレイン凝集変異遺伝子を利用したパーキンソン病モデルマウスの構築. 山口老年総合研究所年報33 2022 ISSM 1348-5237 75-80 山口老年総合研究所

Mikiko Nakamura, Hiroki Kikuta, Yukie Misumi, Ayako Suzuki, Hisashi Hoshida, and Rinji Akada. Triple gene expressions in yeast, Escherichia coli, and mammalian cells by transferring DNA fragments amplified from a mother yeast expression plasmid. Journal of Bioscience and Bioengineering 133, 587-595 (2022 Jun)

 

 

 2021年度

(研究論文)

Kataoka N, Saichana N, Matsutani M, Toyama H, Matsushita K, Yakushi T. 2022. Characterization of three phylogenetically distinct membrane-bound D-gluconate dehydrogenases of Gluconobacter spp. and their biotechnological application for efficient 2-keto-D-gluconate production. Biosci Biotechnol Biochem in press.

Miah R, Nina S, Murate T, Kataoka N, Matsutani M, Ano Y, Matsushita K, Yakushi T. 2022. Dissection and reconstitution provide insights into electron transport in the membrane-bound aldehyde dehydrogenase complex of Gluconacetobacter diazotrophicus. J Bacteriol. 204:e0055821. doi:10.1128/jb.00558-21

Kataoka N, Matsutani M, Murata R, Koga R, Nantapong N, Yakushi T, Matsushita K. 2022. Potassium ion leakage impairs thermotolerance in Corynebacterium glutamicum. J Biosci Bioeng 133:119-125. doi:10.1016/j.jbiosc.2021.10.005.

Schweikert S, Kranz A, Yakushi T, Filipchyk A, Polen T, Etterich H, Bringer S, Bott M. 2021. FNR-Type Regulator GoxR of the Obligatorily Aerobic Acetic Acid Bacterium Gluconobacter oxydans Affects Expression of Genes Involved in Respiration and Redox Metabolism. Appl Environ Microbiol 87:e00195-21. doi: 10.1128/aem.00195-21.

Nguyen TM, Goto M, Noda S, Matsutani M, Hodoya Y, Kataoka N, Adachi O, Matsushita K, Yakushi T. 2021. The 5-Ketofructose Reductase of Gluconobacter sp. Strain CHM43 Is a Novel Class in the Shikimate Dehydrogenase Family. J Bacteriol 203:e0055820. doi: 10.1128/jb.00558-20.

Nakamura K, Nagaki K, Matsutani M, Adachi O, Kataoka N, Ano Y, Theeragool G, Matsushita K, Yakushi T. 2021. Relocation of dehydroquinate dehydratase to the periplasmic space improves dehydroshikimate production with Gluconobacter oxydans strain NBRC3244. Appl Microbiol Biotechnol 105:5883-5894. doi: 10.1007/s00253-021-11476-8.

Matsumoto N, Osumi N, Matsutani M, Phathanathavorn T, Kataoka N, Theeragool G, Yakushi T, Shiraishi Y, Matsushita K. 2021. Thermal adaptation of acetic acid bacteria for practical high-temperature vinegar fermentation. Biosci Biotechnol Biochem 85:1243-1251. doi: 10.1093/bbb/zbab009.

Habe H, Sato Y, Tani H, Matsutani M, Tanioka K, Theeragool G, Matsushita K, Yakushi T. 2021. Heterologous expression of membrane-bound alcohol dehydrogenase-encoding genes for glyceric acid production using Gluconobacter sp. CHM43 and its derivatives. Appl Microbiol Biotechnol 105:6749-6758. doi: 10.1007/s00253-021-11535-0.

Tomoyuki Kosaka, Tatsuya Tsuzuno, Seiki Nishida, Sornsiri Pattanakittivorakul, Masayuki Murata, Isamu Miyakawa, Noppon Lertwattanasakul, Savitree Limtong and Mamoru Yamada: Distinct metabolic flow in response to temperature in thermotolerant Kluyveromyces marxianus. Applied and Environmental Microbiology, doi:10.1128/AEM.02006-21 (Jan, 2022)

Roni Miah, Ayesha Siddiqa, Udvashita Chakraborty, Jamsheda Ferdous Tuli, Noyon Kumar Barman, Aukhil Uddin, Tareque Aziz, Nadim Sharif, Shuvra Kanti Dey, Mamoru Yamada and Ali Azam Talukder:Development of high temperature simultaneous saccharification and fermentation by thermosensitive Saccharomyces cerevisiae and Bacillus amyloliquefaciens. Scientific Reports, 12:3630  https//doi.org/10.1038/s41598-022-07589-3(Jan, 2022)

Koudkeo Phommachan, Chansom Keo-oudone, Mochamad Nurcholis , Nookhao Vongvilaisak , Mingkhuan Chanhming , Vanhnavong Savanhnaly , Somchanh Bounphanmy , Minenosuke Matsutani, Tomoyuki Kosaka, Savitree Limtong, and Mamoru Yamada: Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis. Energies, 15, 561. doi.org/10.3390/en15020561 (Jan, 2022)

Izumi Kumakiri, Yusuke Maruo, Ryotaro Kishibe, Masayuki Murata, Tomoyuki Kosaka, and Mamoru Yamada: Application of Zeolite Membranes to Dehydrate a Bio-Ethanol Solution Produced by High-Temperature Fermentation. Fuels, 2, 533–545. https://doi.org/10.3390/fuels2040031 (Nov 2021)

Masayuki Murata, Keiko Nakamura, Tomoyuki Kosaka, Natsuko Ota, Ayumi Osawa, Ryunosuke Muro, Kazuya Fujiyama, Taku Oshima, Hirotada Mori, Barry L. Wanner and Mamoru Yamada: Cell Lysis Directed by SulA in Response to DNA Damage in Escherichia coli. Int. J. Mol. Sci. 2021, 22, 4535. https://doi.org/10.3390/ijms22094535 (April 2021)

Kikuta, H.; Goto, S., Kondo, M.; Akada, R,; Hoshida, H., Identification of essential intron sequences that enhance gene expression independently of splicing in the yeast Saccharomyces cerevisiae, BBA-Gene Regulatory Mechanisms, 18665, 194784

Kondo, W,; Kitagawa, T,; Hoshida, H,; Akada, R.; Miyakawa, Morphological Changes of Mitochondria and Actin Cytoskeleton in the Yeast Saccharomyces cerevisiae During Diauxic Growth and Glucose Depletion Culture.,Cytologia, accepted.

Abdel-Banat, B. M. A., Hoshida, H., Akada, R., Various short autonomously replicating sequences from the yeast Kluyveromyces marxianus seemingly without canonical consensus., Curr. Res. Microbial Sci. 2, 2021, https://doi.org/10.1016/j.crmicr.2021.100053.

Nakamura, M., Kikuta, H., Misumi, Y., Suzuki, A., Hoshida, H., Akada, R., Triple gene expression in yeast Escherichia coli, and mammalian cells by transferring DNA fragments amplified from a mother yeast expression plasmid, J. Biosci. Bioeng., accepted.

 

(著書)

・Noppon Lertwattanasakul, Mochamad Nurcholis, Nadchanok Rodrussamee, Tomoyuki Kosaka, Masayuki Murata, and Mamoru Yamada: Kluyveromyces marxianus as a platform in synthetic biology for producing useful materials. In Synthetic Biology of Yeasts: Tools and Applications, F. D. Harzevili (ed) Springer, pp 293-335. doi.org/10.1007/978-3-030-89680-5 (2022)

 

(その他)

・Kumakiri I, Yokota M, Tanaka R, Shimada Y, Kiatkittipong W, Lim JW, Murata M, and Yamada M: Process intensification in bio-ethanol production–recent developments in membrane separation. Processe, 9, 1028. https://doi.org/10.3390/pr9061028 (2021)

 

2020年度

(研究論文)

Hoshida H, Kagawa S, Ogami K, and Akada R: Anoxia-induced mitophagy in the yeast Kluyveromyces marxianus. FEMS Yeast Res, doi:10.1093/femsyr/foaa057 (2020)

Fujiwara N, Shibutani S, Sakai Y, Watanabe T, Kitabayashi I, Oshima H, Oshima M, Hoshida H, Akada R, Usui T, Ohama T, and Sato K: Autophagy regulates levels of tumor suppressor enzyme protein phosphatase 6. Cancer Sci, doi:1114371438010.1111/cas.14662 (2020)

Sato T, Hoshida H, and Akada R: Inhibition of Distinct Proline- or N-Acetylglucosamine-Induced Hyphal Formation Pathways by Proline Analogs in Candida albicans. Biomed Res Int. doi:10.1155/2020/7245782 (2020)

Matsumoto N, Matsutani M, Azuma Y, Kataoka N, Yakushi T, and Matsushita K: In vitro thermal adaptation of mesophilic Acetobacter pasteurianus NBRC 3283 generates thermotolerant strains with evolutionary trade-offs. Biosci Biotechnol Biochem. 84:832-841. doi: 10.1080/09168451.2019.1703638 (2020)

Naloka K, Matsushita K, and Theeragool G: Enhanced ultrafine nanofibril biosynthesis of bacterial nanocellulose using a low-cost material by the adapted strain of Komagataeibacter xylinus MSKU 12. Int J Biol Macromol. 150:1113-1120. doi: 10.1016/j.ijbiomac.2019.10.117 (2020)

Yakushi T, Takahashi R, Matsutani M, Kataoka N, Hours RA, Ano Y, Adachi O, and Matsushita K: The membrane-bound sorbosone dehydrogenase of Gluconacetobacter liquefaciens is a pyrroloquinoline quinone-dependent enzyme. Enzyme Microb Technol. 137:109511. doi: 10.1016/j.enzmictec.2020.109511 (2020)

Adachi O, Nguyen TM, Hours RA, Kataoka N, Matsushita K, Akakabe Y, and Yakushi T: 5-Keto-D-fructose production from sugar alcohol by isolated wild strain Gluconobacter frateurii CHM 43. Biosci Biotechnol Biochem. 84(8):1745-1747. doi: 10.1080/09168451.2020.1767500 (2020)

Adachi O, Hours RA, Akakabe Y, Arima H, Taneba R, Tanaka J, Kataoka N, Matsushita K, and Yakushi T: Taro koji of Amorphophallus konjac enabling hydrolysis of konjac polysaccharides to various biotechnological interest. Biosci Biotechnol Biochem. 84:2160-2173. doi: 10.1080/09168451.2020.1787812 (2020)

Sriherfyna FH, Matsutani M, Hirano K, Koike H, Kataoka N, Yamashita T, Nakamaru-Ogiso E, Matsushita K, and Yakushi T: The auxiliary NADH dehydrogenase plays a crucial role in redox homeostasis of nicotinamide cofactors in the absence of the periplasmic oxidation system in Gluconobacter oxydans NBRC3293. Appl Environ Microbiol. 87(2):e02155-20. doi: 10.1128/AEM.02155-20 (2021)

Kataoka N, Hirata K, Matsutani M, Ano Y, Nguyen TM, Adachi O, Matsushita K, and Yakushi T: Three ATP-dependent phosphorylating enzymes in the first committed step of dihydroxyacetone metabolism in Gluconobacter thailandicus NBRC3255. Appl Microbiol Biotechnol. 105(3):1227-1236. doi: 10.1007/s00253-021-11092-6 (2021)

Nguyen TM, Naoki K, Kataoka N, Matsutani M, Ano Y, Adachi O, Matsushita K, and Yakushi T: Characterization of a cryptic, pyrroloquinoline quinone-dependent dehydrogenase of Gluconobacter sp. strain CHM43 Biosci Biotechnol Biochem. 24;85(4):998-1004. doi 10.1093/bbb/zbab005 (2021)

Miah R, Nina S, Murate T, Kataoka N, Matsutani M, Matsushita K, and Yakushi T: Major aldehyde dehydrogenase AldFGH of Gluconacetobacter diazotrophicus is independent of pyrroloquinoline quinone but dependent on molybdopterin for acetic acid fermentation Appl Microbiol Biotechnol 105:2341-2350. doi: 10.1007/s00253-021-11144-x (2021)

 

(著書)

Kataoka N, Matsutani M, and Matsushita K: Respiratory chain and energy metabolism of Corynebacterium glutamicum, In Corynebacterium glutamicum -Biology and Biotechnology-, 2nd edition, Inui, M, & Toyoda, K (Eds). Springer Nature Switzerland AG, Cham, Switzerland, pp.61-85 (2020)

 

(特許)

・特許出願:山田守,村田正之,眞鍋敏朗,長武志;2020-199376「セロビオース分解能を有する酵母」

・特許出願:赤田倫治,星田尚司,中村美紀子;特願2015-156575「ポリペプチド抽出方法」

・特許出願:赤田倫治,藤本哲憲,仲田徳厚,中富正;2020-202035「核酸の増幅方法及びサーマルサイクラー」

・特許出願:足立収生,藥師寿治,片岡尚也,松下一信;2021-001146「D-マンノース異性化酵素及びD-フルクトースの製造方法」

・特許登録:赤田倫治,星田尚司,中村美紀子;2015-156575「ポリペプチド抽出方法」

 

2019年度

(研究論文)

Kosaka T, Nishioka A, Sakurada T, Miura K, Anggarini S, and Yamada M: Enhancement of thermal resistance by metal ions in the thermotolerant Zymomonas mobilis TISTR 548. Front Microbiol, doi:10.3389/fmicb.2020.00502 (2020)

Sakunda A, Masayuki M, Keisuke K, Kosaka T, Kaewta S, Thanonkeo P, Yamada M: Improvement of Thermotolerance of Zymomonas moblis by Genes for Reactive Oxygen Species-Scavenging Enzymes and Heat-Shock Proteins. Front Microbiol, doi:10.3389/fmicb.2019.03073 (2020)

Sumikawa K, Kosaka T, Udo K, Kanesaki Y, Yoshikawa H, and Yamada M: Characteristics of physiology of and genomic mutations in aggregation-enhanced mutants of Methanothermobacter sp. CaT2. Biosci, Biotechnol Biochem, doi: 10.1080/09168451.2019.1709790 (2020)

Nurcholis M, Murata M, Limtong S, Kosaka T, and Yamada M: MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus. Sci Rep, 9(1):9926. doi: 10.1038/s41598-019-46411-5 (2019)

Tien DTK, Phong HX, Yamada M, Toan HT, and Dung NTP: Characterization of newly isolated thermotolerant yeasts and evaluation of their potential for use in Cayratia trifolis wine production. Viet J Sci Technol Eng, 61:68-73 (2019)

Kosaka T, Nakajima Y, Ishii A, Yamashita M, Yoshida S, Murata M, Kato K, Shiromaru Y, Kato S, Kanesaki Y, Yoshikawa H, Matsutani M, Thanonkeo P, and Yamada M: Capacity for survival in global warming: Adaptation of mesophiles to the temperature upper limit. PLoS ONE, 14(6):e0218985. doi: 10.1371/journal.pone.0215614 (2019)

Sumikawa K, Kosaka T, Mayahara N, Matsutani M, Kimoto M, Udo K, and Yamada M: An aggregation-defective mutant of Methanothermobacter sp. CaT2 reveals a unique protein-dependent aggregation. Microbes Environ, 34(3):244-251. doi: 10.1264/jsme2.ME19014 (2019).

Phong HX, Klanrit P, Dung NTP, Yamada M and Thanonkeo P: Isolation and characterization of thermotolerant yeasts for the production of second-generation bioethanol. Ann Microbiol, 69:765-776 (2019)

Nantapong, N, Matsutani, M, Kanchanasin, P, Kataoka, N, Paisrisan, P, Matsushita K, Tanasupawat, S, Corynebacterium suranareeae sp. Nov, a glutamate producing bacterium isolated from soil and its complete genome-based analysis. Int J Syst Evol Microbiol 70(3): 1903-1911. 10.1099/ijsem.0.003993 (2020)

Naloka, K, Yukphan, P, Matsutani, M, Matsushita K, Theeragool, G, Komagataeibacter diospyri sp. Nov, a novel species of thermotolerant bacterial nanocellulose-producing bacterium. Int J Syst Evol Microbiol 70(1): 251-258. 10.1099/ijsem.0.003745 (2020)

Matsutani, M, Matsumoto, N, Hirakawa, H, Shiwa, Y, Yoshikawa, H, Okamoto-Kainuma, A, Ishikawa, M, Kataoka, N, Yakushi T, Matsushita K, Comparative genomic analysis of closely related Acetobacter pasteurianus strains provides evidence of horizontal gene transfer and reveals factors necessary for thermotolerance. J Bacteriol 202(8):e00553-19. 10.1128/jb.00553-19 (2020)

Taweecheep, P, Naloka, K, Matsutani, M, Yakushi T, Matsushita K, Theeragool, G, Superfine bacterial nanocellulose produced by reverse mutations in the bcsC gene during adaptive breeding of Komagataeibacter oboediens. Carbohydr Polym 226: 115243. 10.1016/j.carbpol.2019.115243 (2019)

Taweecheep, P, Naloka, K, Matsutani, M, Yakushi T, Matsushita K, Theeragool, G, In vitro thermal and ethanol adaptations to improve vinegar fermentation at high temperature of Komagataeibacter oboediens MSKU 3. Appl Biochem Biotechnol 189(1): 144-159. 10.1007/s12010-019-03003-3 (2019)

Phathanathavorn, T, Naloka, K, Matsutani, M, Yakushi T, Matsushita K, Theeragool, G, Mutated fabG gene encoding oxidoreductase enhances the cost-effective fermentation of jasmine rice vinegar in the adapted strain of Acetobacter pasteurianus SKU 1108. J Biosci Bioeng 127(6): 690-697. 10.1016/j.jbiosc.2018.12.006 (2019)

Nantapong, N, Murata, R, Trakulnaleamsai, S, Kataoka, N, Yakushi T, Matsushita K, The effect of reactive oxygen species (ros) and ros-scavenging enzymes, superoxide dismutase and catalase, on the thermotolerant ability of Corynebacterium glutamicum. Appl Microbiol Biotechnol 103(13): 5355-5366. 10.1007/s00253-019-09848-2 (2019)

Mahipant, G, Kato, J, Kataoka, N, Vangnai, AS, An alternative genome-integrated method for undomesticated Bacillus subtilis and related species. J Gen Appl Microbiol 65(2): 96-105. 10.2323/jgam.2018.06.001 (2019)

Fujisawa K, Takami T, Nagatomo T, Fukui Y, Hoshida H, Saeki I, Matsumoto, T, Hidaka, I, Yamamoto, N, Sakaida, I, Usefulness of adult medaka fish as a model for the evaluation of alcoholic fatty liver. Alcohol, 77:147-154, doi: 10.1016/j.alcohol.2019.01.005. (2019)

Misumi Y, Nishioka S, Fukuda A, Uemura T, Nakamura M, Hoshida H, Akada R,  YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2-μm circle sequence in cir0 strains of Saccharomyces cerevisiae. Yeast, 36: 249-257 (2019)

Kosaka T, Goda M, Inoue M, Yakushi T, Yamada M: Flagellum-mediated motility in Pelotomaculum thermopropionicum SI. Biosci. Biotechnol. Biochem. 83(7): 1362-1371. doi: 10.1080/09168451.2019.1597618 (2019)

Talukder AA, Adnan N, Siddiqa A, Miah R, Tuli JF, Khan ST, Dey SK, Lertwattanasakul N, Yamada M: Fuel Ethanol Production Using Xylose Assimilating and High Ethanol Producing Thermosensitive Saccharomyces cerevisiae Isolated from Date Palm Juice in Bangladesh. Biocatal. Agri. Biotechnol. doi: 10.1016/j.bcab.2019.101029 (2019)

Pattanakittivorakul S, Lertwattanasakul N, Yamada M, Limtong S, Selection of thermotolerant Saccharomyces cerevisiae for high temperature ethanol production from molasses and increasing ethanol production by strain improvement. Antonie van Leeuwenhoek 112(7):975-990. Doi: 10.1007/s10482-019-01230-6 (2019)

 

(著書)

赤田倫治,中村美紀子,星田尚司3番目のDNA連結反応NHEJ,日本生物工学会和文誌 98(1), 23-27 (2020)

 

(特許)

山田守高坂智之;特願2020-37036「耐熱性および培養ストレス耐性を備えたエタノール生産酵母を生産する方法」,出願日:202034

赤田倫治,柳井和弥,星田尚司;特願2020-026375「タグペプチド及びポリペプチドの精製方法」,出願日:2020219日

足立収生,薬師寿治片岡尚也松下一信;特願2019-097279「アミンの酸化方法」,出願日:2019524

 

(その他)

今井, 山田;宇部市バイオマス産業都市構想の事業化プロジェクトに関する提言書; 2020年3

 

 

2018年度

(研究論文)

Kosaka T, Goda M, Inoue M, Yakushi T, Yamada M. Flagellum-mediated motility in Pelotomaculum thermopropionicum SI. Biosci. Biotechnol. Biochem. 83(7):1362-1371. doi: 10.1080/09168451.2019.1597618 (2019)

Talukder AA, Adnan N, Siddiqa A, Miah R, Tuli JF, Khan ST, Dey SK, Lertwattanasakul N, Yamada M: Fuel Ethanol Production Using Xylose Assimilating and High Ethanol Producing Thermosensitive Saccharomyces cerevisiae Isolated from Date Palm Juice in Bangladesh, Biocatal. Agri. Biotechnol. doi: 10.1016/j.bcab.2019.101029 (2019)

Pattanakittivorakul S, Lertwattanasakul N, Yamada M, Limtong S. Selection of thermotolerant Saccharomyces cerevisiae for high temperature ethanol production from molasses and increasing ethanol production by strain improvement. Antonie van Leeuwenhoek 112(7):975-990. Doi: 10.1007/s10482-019-01230-6 (2019)

Samappito J, Yamada M, Klanrit P, Thanonkeo P. Characterization of a thermo-adapted strain of Zymomonas mobilis for ethanol production at high temperature. 3 Biotech., 8(11):474. Doi: org/10.1007/s13205-018-1493-7 (2018)

Nurcholis M, Nitiyon S, Suprayogi, Rodrussamee N, Lertwattanasakul N, Limtong S, Kosaka T, Yamada M. Functional analysis of Mig1 and Rag5 as expressional regulators in thermotolerant yeast Kluyveromyces marxianus. Appl. Microbiol. Biotechnol., 103(1):395-410.DOI:10.1007/s00253-018-9462-y (2018)  

Rodrussamee N, Sattayawat P, Yamada M. Highly efficient conversion of xylose to ethanol without glucose repression by newly isolated thermotolerant Spathaspora passalidarum CMUWF1-2. BMC Microbiol. 18:73. DOI: 10.1186/s12866-018-1218-4 (2018)

Yakushi T, Terada Y, Ozaki S, Kataoka N, Akakabe Y, Adachi O, Matsutani M, Matsushita K. Aldopentoses as new substrates for the membrane-bound, pyrroloquinoline quinone-dependent glycerol (polyol) dehydrogenase of Gluconobacter sp. Appl. Microbiol. Biotechnol. 102(7):3159-3171 (2018) 2018 Apr; doi: 10.1007/s00253-018-8848-1.

Matsumoto N, Hattori H, Matsutani M, Matayoshi C, Toyama H, Kataoka N, Yakushi T, Matsushita K. A single-nucleotide insertion in a drug transporter gene induces a thermotolerant phenotype of Gluconobacter frateurii by increasing the NADPH/ NADP+ ratio via metabolic change. Appl. Environ. Microbiol. 84 (10) e00354-18 (2018) doi: 10.1128/AEM.00354-18.

Yakushi T, Fukunari S, Kodama T, Matsutani M, Nina S, Kataoka N, Theeragool G, and Matsushita K. Role of a membrane-bound aldehyde dehydrogenase complex AldFGH in acetic acid fermentation with Acetobacter pasteurianus SKU1108. Appl. Microbiol. Biotechnol. 102(10) 4549-4561 (2018) 2018, doi: 10.1007/s00253-018-8940-6.

Naloka K. Yukphan P, Matsushita K, Theeragool G, Molecular taxonomy and characterization of thermotolerant Komagataeibacter species for bacterial nanocellulose production at high temperatures. Chiang Mai Journal of Science 45 (4) 1610-1622 (2018)

Theeragool, G., Pitiwittayakul, N., Matsutani, M., Matsushita, K, Disruption of the groEL gene revealed a physiological role for chaperonin in the thermotolerant acetic acid bacterium, Acetobacter pasteurianus SKU1108. Chiang Mai Journal of Science 45 (4), 1623-1633 (2018)

Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Wada M, Yokota A, Matsushita K, Engineering of Corynebacterium glutamicum as a prototrophic pyruvate-producing strain: Characterization of a ramA-deficient mutant and its application for metabolic engineering. Biosci. Biotechnol. Biochem. 83(2):372-380 (2019) 2019 Feb; doi: 10.1080/09168451.2018.1527211.

Matsutani M, Hirakawa H, Sriherfyna FH, Kataoka N, Yakushi T, Matsushita K, Diversity of NADH dehydrogenase in acetic acid bacteria; adaptation to modify their phenotype through gene expansion, losses and neo functionalization. Microbiology 165(3) 287-291(2019) 2019 Mar.

Misumi Y, Nishioka S, Fukuda A, Uemura T, Nakamur, M, Hoshida H, Akada R, YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2-μm circle sequence in cir0 strains of Saccharomyces cerevisiae, Yeast, 36:249-257, 2019, doi: 10.1002/yea.3371.

Fujisawa K, Takami T, Nagatomo T, Fukui Y, Hoshida H, Saeki I, Matsumoto T, Hidaka I, Yamamoto N, Sakaida I, Usefulness of adult medaka fish as a model for the evaluation of alcoholic fatty liver, Alcohol, 77:147-154, 2019, doi: 10.1016/j.alcohol.2019.01.005.

Nakamura M, Aihara J, Hoshida H, Akada R, Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products, Mol. Biotechnol., 60:912-923, 2018, doi: 10.1007/s12033-018-0123-2.

 

(著書

・Kosaka T, Lertwattanasakul N, Rodrussamee N, Nurcholis M, Dung NTP, Keo-Oudone C, Murata M, Götz P, Theodoropoulos C, Suprayogi, Maligan JM, Limtong S, Yamada M. Potential of thermotolerant ethanologenic yeasts isolated from ASEAN countries and their application in high-temperature fermentation. In Fuel Ethanol Production from Sugarcane. Thalita Peixoto Basso and Luiz Carlos Basso (eds) ISBN: 978-1-78984-937-0 (print) 978-1-78984-937-7 (online) IntechOpen, pp121-154 (2019)

・赤田倫治,中村美紀子,星田尚司,「耐熱性酵母Kluyveromyces marxianusを用いた物質生産と育種技術」,pp. 24-32,酵母菌・麹菌・乳酸菌の産業応用展開,シーエムシー出版,2018年

 

 

 2017年度

 (研究論文)

Masayuki Murata, Ayana Ishii, Hiroko Fujimoto, Kaori Nishimura, Tomoyuki Kosaka, Hirotada Mori, Mamoru Yamada: Update of thermotolerant genes essential for survival at a critical high temperature in Escherichia coli. PLoS ONE, 0189487 (2018) doi.org/10.1371/journal.pone.

Nuntipa Khumkarjorn, Sudarat Thanonkeo, Mamoru Yamada, Preekamol Klanrit and Pornthap Thanonkeo: Agrobacterium-mediated transformation of Dendrobium orchid with the flavanone 3-hydroxylase gene, Turkish J. Botany, 41:442-454 (2017) doi: 10.3906/bot-1701-13.

Kannikar Charoensuk, Tomoko Sakurada, Amina Tokiyama, Masayuki Murata, Tomoyuki Kosaka, Pornthap Thanonkeo, Mamoru Yamada: Thermotolerant genes essential for survival at a critical high temperature in thermotolerant ethanologenic Zymomonas mobilis TISTR 548. Biotechnol Biofuels, 10:204 (2017) doi: 10.1186/s13068-017-0891-0.

Nuntipa Khumkarjorn, Sudarat Thanonkeo, Mamoru Yamada, Pornthap Thanonkeo: Cloning and expression analysis of a flavanone 3-hydroxylase gene in Ascocenda orchid. J. Plant Biochem. Biotechnol. 26:179-190 (2017) DOI 10.1007/s13562-016-0379-1.

Patumporn Sottirattanapan, Kasem Nantachai, Sakda Daduang, Toshitaka Funahashi, Mamoru Yamada: Purification and characterization of amylase from roots of Paederia foetida Linn. Biocatalysis and Agricultural Biotechnology, 10:329-335 (2017)

Srianta Ignatius, Elok Zubaidah, Teti Estiasih, Yoshihito Iuchi, Harijono, Mamoru Yamada: Antioxidant activity of pigments derived from Monascus purpureus-fermented rice, corn, and sorghum, International Food Research Journal, 24: 1186-1191 (June 2017)

Matsutani M, Nantapong N, Murata R, Paisrisan P, Hirakawa H, Kataoka N, Yakushi T, Matsushita K. Complete genome sequencing of newly isolated thermotolerant Corynebacterium glutamicum N24 provides a new insights into its thermotolerant phenotype. J Biotechnol. 247: 29-33 (2017) 10 April 2017 doi: 10.1016/j.jbiotec.2017.02.025.

Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Production of 1,3-diols in Escherichia coli. Bioresour Technol. 245(Pt B):1538-154, 2017 Dec;doi:10.1016/j.biortech.2017.05.082.

Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Butyrate production under aerobic growth conditions by engineered Escherichia coli. J Biosci Bioeng. 123(5):562-568, 2017 May; doi: 10.1016/ j.jbiosc.2016.12.008.

Yoshida Y, Kuroiwa H, Shimada T, Yoshida M, Ohnuma M, Fujiwara T, Imoto Y, Yagisawa F, Nishida K, Hirooka S, Misumi O, Mogi Y, Akakabe Y, Matsushita K, Kuroiwa T. Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments. Proc Natl Acad Sci USA 114 (50): 13284-13289, 2017 Dec 12; doi: 10.1073/pnas.1715008114.

Yakushi T, Komatsu K, Matsutani M, Kataoka N, Vangnai AS, Toyama H, Adachi O, Matsushita K. Improved heterologous expression of the membrane-bound quinoprotein quinate dehydrogenase from Gluconobacter oxydans. Protein Expr Purif. 145:100-107, 2018 May; doi: 10.1016/j.pep.2018.01.007.

Yakushi T, Terada Y, Ozaki S, Kataoka N, Akakabe Y, Adachi O, Matsutani M, Matsushita K. Aldopentoses as new substrates for the membrane-bound, pyrroloquinoline quinone-dependent glycerol (polyol) dehydrogenase of Gluconobacter sp. Appl Microbiol Biotechnol. 102(7):3159-3171, 2018 Apr; doi: 10.1007/s00253- 018-8848-1. 

Matsumoto N, Hattori H, Matsutani M, Matayoshi C, Toyama H, Kataoka N, Yakushi T, Matsushita K. A single-nucleotide insertion in a drug transporter gene induces a thermotolerant phenotype  of Gluconobacter frateurii by increasing the NADPH/ NADP+ ratio via metabolic change.Appl Environ Microbiol. 2018 Mar 16. pii: AEM.00354-18. doi: 10.1128/AEM.00354-18.

Yakushi T, Fukunari S, Kodama T, Matsutani M, Nina S, Karaoke N, Theeragool G, and Matsushita K. Role of a membrane-bound aldehyde dehydrogenase complex AldFGH in acetic acid fermentation with Acetobacter pasteurianus SKU1108. Appl. Microbiol. Biotechnol. 2018 in press

Hoshida H, Kidera K, Takishita R, Fujioka N, Fukagawa T, Akada R. Enhanced production of extracellular inulinase by the yeast Kluyveromyces marxianus in xylose catabolic state. J. Biosci. Bioeng. (in press).

Ortiz-Merino RA, Varela JA, Coughlan AY, Hoshida H, da Silveira WB, Wilde C, Kuijpers NGA, Geertman J-M, Wolfe1 KH and Morrissey JP. Ploidy Variation in Kluyveromyces marxianus Separates Dairy and Non-dairy Isolates. Front. Genet., 9: 94 (2018)

 

(著書) 

・Hoshida H and Akada R, High-temperature bioethanol fermentation by conventional and nonconventional yeasts, "Biotechnology of yeasts and filamentous fungi", A. Sibirny (edt.), Springer, pp. 39-62 (2017)

・Hoshida H, Yarimizu T, Akada R, Selection of error-less synthetic genes in yeast, "Synthetic DNA", Hughes RA (Edt.), Springer, pp. 237-246 (2017)

 

2016年度

(研究論文)

Ano Y, Hours RA, Akakabe Y, Kataoka N, Yakushi T, Matsushita K, Adachi O. Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose. Biosci Biotechnol Biochem, 81: 411-418 (2017) doi: 10.1080/ 09168451.2016.1254535.

Hoshida H, Kondo M, Kobayashi T, Yarimizu T, Akada R, 5´-UTR introns enhance protein expression in the yeast Saccharomyces cerevisiae. Appl Mcrobiol Biotechnol 101: 241 (2017) doi: 10.1007/ s00253-016-7891-z.

Ignatius S, Zubaidah E, Estiasih T, Yamada M, Harijono H: Comparison of Monascus purpureus growth, pigment production and composition on different cereal substrates with solid state fermentation. Biocatalysis and Agricultural Biotechnology, 7:181-186 (2016) doi:10.1016/j.bcab.2016.05.011

Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Butyrate production under aerobic growth conditions by engineered Escherichia coli. J Biosci Bioeng, 123: 562-568 (2017) doi: 10.1016/ j.jbiosc.2016.12.008.

Matsushita K, Azuma Y, Kosaka T, Yakushi T, Hoshida H, Akada R, Yamada M. Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations. Biosci Biotechnol Biochem, 80: 655-668 (2016) doi: 10.1080/09168451.2015.1104235.

Matsutani M, Hirakawa H, Hiraoka E, Theeragool G, Yakushi T, Matsushita K. Complete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, Acetobacter pasteurianus SKU1108, Provide a New Insight into Thermotolerance. Microbes Environ, 31: 395-400 (2016) doi: 10.1264/jsme2.ME16023.

Nakamura M, Suzuki A, Yarimizu T, Hoshida H, Akada R, A PCR protocol for amplification of DNA from complex templates. Accounts of Biotechnology Research, 3: 28 (2016)

Nuanpeng S, Thanonkeo S, Yamada M, Thanonkeo P.: Ethanol production from sweet sorghum juice at high temperatures using a newly isolated thermotolerant yeast Saccharomyces cerevisiae DBKKI Y-53. Energies 9(4), 253 (2016); DOI 10.3390/en9040253

Sainz F, Jesús Torija M, Matsutani M, Kataoka N, Yakushi T, Matsushita K, Mas A. Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains. Front Microbiol, 7: 1358 (2016) doi: 10.3389/fmicb.2016.01358.

Suprayogi, Nurcholis M, Murata M, Lertwattanasakul N, Kosaka T, Rodrussamee N, Limtong S, Yamada M. Characteristics of kanMX4-inserted mutants that exhibit 2-deoxyglucose resistance in thermotolerant yeast Kluyveromyces marxianus. The Open Biotechnology Journal, 10: 208-222 (2016) doi: 10.2174/18740707016100100208

 

(著書)

Matsushita K and Matsutani M: Chapter 7. Distribution, evolution and physiology of oxidative fermentation, "Acetic acid bacteria: Ecology and Physiology", K. Matsushita et al. (eds.), Springer, pp. 159-178 (2016) ISBN 978-4-431-55931-3.

・Adachi O & Yakushi T: Chapter 13. Membrane-bound dehydrogenases of acetic acid bacteria "Acetic acid bacteria: Ecology and Physiology", K. Matsushita et al. (eds.), Springer, pp. 273-297 (2016) ISBN 978-4-431-55931-3.

 

(その他)

・村田正之,スカンヤ ニチヨン,チャンソム ケオオウドン,スプラヨギ,サビトリー リムトン,高坂 智之,山田 守:第二世代バイオマス(セルロース系バイオマス)のエタノール変換・生産技術 化学工業,67: 519-528 (2016)

 

(特許)

・特許出願:中村美紀子,赤田倫治,星田尚司;特願2015-220058「タグペプチド」

・特許出願:赤田倫治,星田尚司,中村美紀子;特願2015-156575「ポリペプチド抽出方法」

・特許出願:中村美紀子,赤田倫治,星田尚司;特願2015-007674「ターミネータ配列とプロモータ配列とを順次備えた線状二本鎖DNA」

・特許登録:山田 守, 藤元奈保子;特許 5769187「セルロース原料の分解能を有する微生物、及びこれを用いたセルロース原料の分解処理」(登録日:平成27年7月3日)

・国際出願:山田 守, スカンヤ ニチョン, ケオ-ウドネ チャンソム 国際出願番号:PCT/JP2015/002175「キシロース資化能及びエタノール生産能を有する酵母」(国際出願日:2015年4月22日)

 

2015年度

(研究論文)

Charoenyingcharoen P, Matsutani M, Yakushi T, Theeragool G, Yukphan P, Matsushita K. A functionally critical single nucleotide polymorphism in the gene encoding the membrane-bound alcohol dehydrogenase found in ethanol oxidation-deficient Gluconobacter thailandicus. Gene 567: 201-207 (2015) doi: 10.1016/j.gene.2015.04.080.

Charoensopharat K, Thanonkeo P, Thanonkeo S, Yamada M. Ethanol production from Jerusalem artichoke tubers at high temperature by newly isolated thermotolerant inulin-utilizing yeast Kluyveromyces marxianus using consolidated bioprocessing. Antonie Van Leeuwenhoek, 108: 173-190 (2015) doi: 10.1007/s10482-015-0476-5.

Kataoka N, Matsutani M, Yakushi T, Matsushita K. Efficient Production of 2,5-Diketo-d-Gluconate via Heterologous Expression of 2-Ketogluconate Dehydrogenase in Gluconobacter japonicus. Appl Environ Microbiol. 81: 3552-3560 (2015) doi: 10.1128/AEM.04176-14.

Kataoka N, Vangnai AS, Pongtharangkul T, Tajima T, Yakushi T, Matsushita K, Kato J. Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli. J Biotechnol. 204: 25-32 (2015) doi: 10.1016/j.jbiotec.2015.03.027.

Kawai M, Higashiura N, Hayasaki K, Okamoto N, Takami A, Hirakawa H, Matsushita K, Azuma Y. Complete genome and gene expression analyses of Asaia bogorensis reveal unique responses to culture with mammalian cells as a potential opportunistic human pathogen. DNA Res. 22: 357-366 (2015) doi: 10.1093/dnares/dsv018.

Keo-oudone C, Nitiyon S, Sotitham P, Tani A, Lertwattanasakul N, Yuangsaard N, Bounphanmy S, Limtong S, Yamada M. Isolation and characterization of thermotolerant ethanol-fermenting yeasts from Laos and application of whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) analysis for their quick identification. African Journal of Biotechnology, 15: 153-164 (2016) doi: 10.5897/AJB2015.14984.

Kitagawa T, Okita H, Baron B, Tokuda K, Nakamura M, Wang Y, Akada J, Hoshida H, Akada R, Kuramitsu Y, Nakamura K, Mutant screening for oncogenes of Ewing's sarcoma using yeast. Appl Microbiol Biotechnol, 99: 3737 (2015) doi: 10.1007/s00253-015-6621-2.

Matsutani M, Ito K, Azuma Y, Ogino H, Shirai M, Yakushi T, Matsushita K. Adaptive mutation related to cellulose producibility in Komagataeibacter medellinensis (Gluconacetobacter xylinus) NBRC 3288. Appl Microbiol Biotechnol. 99: 7229-7240 (2015) doi: 10.1007/s00253-015-6598-x.

Murata M, Nitiyon S, Lertwattanasakul N, Sootsuwan K, Kosaka T, Thanonkeo P, Limtong S, Yamada M. High-temperature Fermentation Technology for Low-cost Bioethanol. Journal of the Japan Institute of Energy, 94: 1154-1162 (2015) doi: 10.3775/jie.94.1154.

Nakamura M, Suzuki A, Akada J, Tomiyoshi K, Hoshida H, Akada R, End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter. Mol Biotechnol, 57: 1018 (2015) doi: 10.1007/s12033-015-9890-1.

Nakamura M, Suzuki A, Akada J, Yarimizu T, Iwakiri R, Hoshida H, Akada R, A Novel Terminator Primer and Enhancer Reagents for Direct Expression of PCR-Amplified Genes in Mammalian Cells. Mol Biotechnol, 57: 767 (2015) doi: 10.1007/s12033-015-9870-5.

Nitiyon S, Keo-Oudone C, Murata M, Lertwattanasakul N, Limtong S, Kosaka T, Yamada M. Efficient conversion of xylose to ethanol by stress-tolerant Kluyveromyces marxianus BUNL-21. Springerplus, 5: 185 (2016) doi: 10.1186/s40064-016-1881-6.

Saichana N, Matsushita K, Adachi O, Frébort I, Frébortová J. Acetic acid bacteria: A group of bacteria with versatile biotechnological applications. Biotechnol Adv. 33, 1260-1271 (2015) doi: 10.1016/j.biotechadv. 2014.12.001.

Saichana N, Tanizawa K, Pechoušek J, Novák P, Yakushi T, Toyama H, Frébortová J. PqqE from Methylobacterium extorquens AM1: a radical S-adenosyl-l-methionine enzyme with an unusual tolerance to oxygen. J Biochem. 159: 87-99 (2016) doi: 10.1093/jb/mvv073.

Soemphol W, Tatsuno M, Okada T, Matsutani M, Kataoka N, Yakushi T, Matsushita K. A novel Na+(K+)/H+ antiporter plays an important role in the growth of Acetobacter tropicalis SKU1100 at high temperatures via regulation of cation and pH homeostasis. J Biotechnol. 211: 46-55 (2015) doi: 10.1016/ j.jbiotec.2015.06.397.

Suprayogi, Nguyen MT, Lertwattanasakul N, Rodrussamee N, Limtong S, Kosaka T, Yamada M. A Kluyveromyces marxianus 2-deoxyglucose-resistant mutant with enhanced activity of xylose utilization. Int Microbiol, 18: 235-244 (2015) doi: 10.2436/20.1501.01.255.

Suzuki A, Fujii H, Hoshida H, Akada R., Gene expression analysis using strains constructed by NHEJ-mediated one-step promoter cloning in the yeast Kluyveromyces marxianus. FEMS Yeast Res, 15, fov059 (2015) doi: 10.1093/femsyr/fov059.

Talukder AA, Sujon SI, Hossain MM, Gomes DJ, Yamada M. Production of Bioethanol at High Temperature from Tari. AiM, 05: 325-335 (2015) doi: 10.4236/aim.2015.55033.

Yamamoto H, Shima T, Yamaguchi M, Mochizuki Y, Hoshida H, Kakuta S, Kondo-Kakuta C, Noda NN, Inagaki F, Itoh T, Akada R, Ohsumi Y, The Thermotolerant Yeast Kluyveromyces marxianus Is a Useful Organism for Structural and Biochemical Studies of Autophagy. J Biol Chem, 290: 29506 (2015) doi: 10.1074/jbc.M115.684233.

Yarimizu T, Nakamura M, Hoshida H, Akada R, Screening of accurate clones for gene synthesis in yeast. J Biosci Bioeng, 119: 251 (2014) doi: 10.1016/j.jbiosc.2014.08.006.

 

 

(その他)

村田正之, 高坂智之, サビトリー リムトン, 山田 守:省エネ型エタノール発酵新技術とセルロース系バイオマス変換基盤技術; 化学工業, 1月号: 1-6 (2015)

・山田 守, 赤田 倫治, 高坂 智之, 東 慶直, 星田 尚司, 松下 一信:耐熱性発酵微生物の耐熱性を付与する分子機構,化学と生物,53 (11): 763-773 (2015)

 

2014年度

(研究論文)

Fujioka Y, Suzuki SW, Yamamoto H, Kondo-Kakuta C, Kimura Y, Hirano H, Akada R, Inagaki F, Ohsumi Y, Noda NN, Structural basis of starvation-induced assembly of the autophagy initiation complex. Nat Struct Mol Biol, 21: 513 (2014) doi: 10.1038/nsmb.2822

Kataoka N, Vangnai AS, Ueda H, Tajima T, Nakashimada Y, Kato J. Enhancement of (R)-1,3-butanediol production by engineered Escherichia coli using a bioreactor system with strict regulation of overall oxygen transfer coefficient and pH. Biosci Biotechnol Biochem, 78 (4): 695-700 (2014)

Kawai S, Yakushi T, Matsushita K, Kitazumi Y, Shirai O, Kano K. Role of a non-ionic surfactant in direct electron transfer-type bioelectrocatalysis by fructose dehydrogenase. Electrochimica Acta 152, 19-24 (2015) doi:10.1016/j.electacta. 2014.11. 113

Kosaka T, Toh H, Fujiyama A, Sakaki Y, Watanabe K, Meng XY, Hanada S, Toyoda A. Physiological and genetic basis for self‐aggregation of a thermophilic hydrogenotrophic methanogen, Methanothermobacter strain CaT2. Environmental Microbiology Reports, 6: 268-277 (2014) doi: 10.1111/1758-2229.12128.

Lertwattanasakul N, Kosaka T, Hosoyama A, Suzuki Y, Rodrussamee N, Matsutani M, Murata M, Fujimoto N, Suprayogi, Tsuchikane K, Limtong S, Fujita N, Yamada M. Genetic basis of the highly efficient yeast Kluyveromyces marxianus: complete genome sequence and transcriptome analyses. Biotechnology for Biofuels, 8: 47 (2015) doi: 10.1186/s13068-015-0227-x.

Matsutani M, Fukushima K, Kayama C, Arimitsu M, Hirakawa H, Toyama H, Adachi O, Yakushi T, Matsushita K.  Replacement of a terminal cytochrome c oxidase by ubiquinol oxidase during the evolution of acetic acid bacteria. Biochim Biophys Acta. 1837: 1810-20 (2014) doi: 10.1016/j.bbabio.2014.05.355.

Matsutani M, Suzuki H, Yakushi T, Matsushita K. Draft genome sequence of Gluconobacter thailandicus NBRC 3257. Stand Genomic Sci. 9: 614-623 (2014) doi: 10.4056/sigs.4778605.

Nishikura-Imamura S, Matsutani M, Insomphun C, Vangnai AS, Toyama H, Yakushi T, Abe T, Adachi O, Matsushita K. Overexpression of a type II 3-dehydroquinate dehydratase enhances the biotransformation of quinate to 3-dehydroshikimate in Gluconobacter oxydans. Appl Microbiol Biotechnol. 98: 2955-2963 (2014) doi: 10.1007/s00253-013-5439-z.

Yarimizu T, Nakamura M, Hoshida H, Akada R, Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus, Microbial Cell Fact, 14: 20 (2015) doi: 10.1186/s12934-015-0203-y.

 

(著書)

・Nakamura M, Suzuki A, Hoshida H, Akada R, Minimum GC-rich sequences for overlap extension PCR and primer annealing, "Methods Mol Biol series: DNA Cloning and Assembly Methods", Valla S and Lale R (eds.), Springer, pp. 165-181 (2014) ISBN: 978-1-62703-763-1.

Hoshida H, Yarimizu T, Akada R, Selection of Error-Less Synthetic Genes in Yeast. "Methods Mol Biol series: Synthetic DNA Cloning", Hughes RA (eds.), Springer, pp. 237-246 (2014) ISBN: 978-1-4939-6341-6

 

(特許)

・山田 守, スカンヤ ニチョン;特開2015-73508「キシロース資化能を有するエタノール生産酵母を生産する方法」(出願日:2014年4月28日)

・星田尚司,赤田倫治:特願2014-158646「高発現プロモーター」国立大学法人山口大学,平成26年8月4日

・山田 守, スカンヤ ニチョン, ケオ-ウドネ チャンソム:特願2014-092206「キシロース資化能及びエタノール生産能を有する酵母」山口大学(2014年4月28日出願)

・赤田倫治,星田尚司,山田 守,村田正之,高坂智之,松下一信,薬師寿治,ナンタポン・ナワラート,東慶直:特願2014-159048「耐熱性微生物の作製方法」山口大学・近畿大学(2014年8月4日出願)

 

(著書)