発酵微生物部門の研究業績

研究論文(著書,その他)

 

2018年度

(研究論文 

Kosaka T, Goda M, Inoue M, Yakushi T, Yamada M. Flagellum-mediated motility in Pelotomaculum thermopropionicum SI. Biosci. Biotechnol. Biochem. 83(7):1362-1371. doi: 10.1080/09168451.2019.1597618 (2019)

Talukder AA, Adnan N, Siddiqa A, Miah R, Tuli JF, Khan ST, Dey SK, Lertwattanasakul N, Yamada M: Fuel Ethanol Production Using Xylose Assimilating and High Ethanol Producing Thermosensitive Saccharomyces cerevisiae Isolated from Date Palm Juice in Bangladesh, Biocatal. Agri. Biotechnol. doi: 10.1016/j.bcab.2019.101029 (2019)

Pattanakittivorakul S, Lertwattanasakul N, Yamada M, Limtong S. Selection of thermotolerant Saccharomyces cerevisiae for high temperature ethanol production from molasses and increasing ethanol production by strain improvement. Antonie van Leeuwenhoek 112(7):975-990. Doi: 10.1007/s10482-019-01230-6 (2019)

Samappito J, Yamada M, Klanrit P, Thanonkeo P. Characterization of a thermo-adapted strain of Zymomonas mobilis for ethanol production at high temperature. 3 Biotech., 8(11):474. Doi: org/10.1007/s13205-018-1493-7 (2018)

Nurcholis M, Nitiyon S, Suprayogi, Rodrussamee N, Lertwattanasakul N, Limtong S, Kosaka T, Yamada M. Functional analysis of Mig1 and Rag5 as expressional regulators in thermotolerant yeast Kluyveromyces marxianus. Appl. Microbiol. Biotechnol., 103(1):395-410.DOI:10.1007/s00253-018-9462-y (2018)  

Rodrussamee N, Sattayawat P, Yamada M. Highly efficient conversion of xylose to ethanol without glucose repression by newly isolated thermotolerant Spathaspora passalidarum CMUWF1-2. BMC Microbiol. 18:73. DOI: 10.1186/s12866-018-1218-4 (2018)

Yakushi T, Terada Y, Ozaki S, Kataoka N, Akakabe Y, Adachi O, Matsutani M, Matsushita K. Aldopentoses as new substrates for the membrane-bound, pyrroloquinoline quinone-dependent glycerol (polyol) dehydrogenase of Gluconobacter sp. Appl. Microbiol. Biotechnol. 102(7):3159-3171 (2018) 2018 Apr; doi: 10.1007/s00253-018-8848-1.

Matsumoto N, Hattori H, Matsutani M, Matayoshi C, Toyama H, Kataoka N, Yakushi T, Matsushita K. A single-nucleotide insertion in a drug transporter gene induces a thermotolerant phenotype of Gluconobacter frateurii by increasing the NADPH/ NADP+ ratio via metabolic change. Appl. Environ. Microbiol. 84 (10) e00354-18 (2018) doi: 10.1128/AEM.00354-18.

Yakushi T, Fukunari S, Kodama T, Matsutani M, Nina S, Kataoka N, Theeragool G, and Matsushita K. Role of a membrane-bound aldehyde dehydrogenase complex AldFGH in acetic acid fermentation with Acetobacter pasteurianus SKU1108. Appl. Microbiol. Biotechnol. 102(10) 4549-4561 (2018) 2018, doi: 10.1007/s00253-018-8940-6.

Naloka K. Yukphan P, Matsushita K, Theeragool G, Molecular taxonomy and characterization of thermotolerant Komagataeibacter species for bacterial nanocellulose production at high temperatures. Chiang Mai Journal of Science 45 (4) 1610-1622 (2018)

Theeragool, G., Pitiwittayakul, N., Matsutani, M., Matsushita, K, Disruption of the groEL gene revealed a physiological role for chaperonin in the thermotolerant acetic acid bacterium, Acetobacter pasteurianus SKU1108. Chiang Mai Journal of Science 45 (4), 1623-1633 (2018)

Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Wada M, Yokota A, Matsushita K, Engineering of Corynebacterium glutamicum as a prototrophic pyruvate-producing strain: Characterization of a ramA-deficient mutant and its application for metabolic engineering. Biosci. Biotechnol. Biochem. 83(2):372-380 (2019) 2019 Feb; doi: 10.1080/09168451.2018.1527211.

Matsutani M, Hirakawa H, Sriherfyna FH, Kataoka N, Yakushi T, Matsushita K, Diversity of NADH dehydrogenase in acetic acid bacteria; adaptation to modify their phenotype through gene expansion, losses and neo functionalization. Microbiology 165(3) 287-291(2019) 2019 Mar.

Misumi Y, Nishioka S, Fukuda A, Uemura T, Nakamur, M, Hoshida H, Akada R, YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2-μm circle sequence in cir0 strains of Saccharomyces cerevisiae, Yeast, 36:249-257, 2019, doi: 10.1002/yea.3371.

Fujisawa K, Takami T, Nagatomo T, Fukui Y, Hoshida H, Saeki I, Matsumoto T, Hidaka I, Yamamoto N, Sakaida I, Usefulness of adult medaka fish as a model for the evaluation of alcoholic fatty liver, Alcohol, 77:147-154, 2019, doi: 10.1016/j.alcohol.2019.01.005.

Nakamura M, Aihara J, Hoshida H, Akada R, Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products, Mol. Biotechnol., 60:912-923, 2018, doi: 10.1007/s12033-018-0123-2.

 

(著書

Kosaka T, Lertwattanasakul N, Rodrussamee N, Nurcholis M, Dung NTP, Keo-Oudone C, Murata M, Götz P, Theodoropoulos C, Suprayogi, Maligan JM, Limtong S, Yamada M. Potential of thermotolerant ethanologenic yeasts isolated from ASEAN countries and their application in high-temperature fermentation. In Fuel Ethanol Production from Sugarcane. Thalita Peixoto Basso and Luiz Carlos Basso (eds) ISBN: 978-1-78984-937-0 (print) 978-1-78984-937-7 (online) IntechOpen, pp121-154 (2019)

赤田倫治,中村美紀子,星田尚司,「耐熱性酵母Kluyveromyces marxianusを用いた物質生産と育種技術」,pp. 24-32,酵母菌・麹菌・乳酸菌の産業応用展開,シーエムシー出版,2018年

 

 

 2017年度

 (研究論文)

Masayuki Murata, Ayana Ishii, Hiroko Fujimoto, Kaori Nishimura, Tomoyuki Kosaka, Hirotada Mori, Mamoru Yamada: Update of thermotolerant genes essential for survival at a critical high temperature in Escherichia coli. PLoS ONE, 0189487 (2018) doi.org/10.1371/journal.pone.

Nuntipa Khumkarjorn, Sudarat Thanonkeo, Mamoru Yamada, Preekamol Klanrit and Pornthap Thanonkeo: Agrobacterium-mediated transformation of Dendrobium orchid with the flavanone 3-hydroxylase gene, Turkish J. Botany, 41:442-454 (2017) doi: 10.3906/bot-1701-13.

Kannikar Charoensuk, Tomoko Sakurada, Amina Tokiyama, Masayuki Murata, Tomoyuki Kosaka, Pornthap Thanonkeo, Mamoru Yamada: Thermotolerant genes essential for survival at a critical high temperature in thermotolerant ethanologenic Zymomonas mobilis TISTR 548. Biotechnol Biofuels, 10:204 (2017) doi: 10.1186/s13068-017-0891-0.

Nuntipa Khumkarjorn, Sudarat Thanonkeo, Mamoru Yamada, Pornthap Thanonkeo: Cloning and expression analysis of a flavanone 3-hydroxylase gene in Ascocenda orchid. J. Plant Biochem. Biotechnol. 26:179-190 (2017) DOI 10.1007/s13562-016-0379-1.

Patumporn Sottirattanapan, Kasem Nantachai, Sakda Daduang, Toshitaka Funahashi, Mamoru Yamada: Purification and characterization of amylase from roots of Paederia foetida Linn. Biocatalysis and Agricultural Biotechnology, 10:329-335 (2017)

Srianta Ignatius, Elok Zubaidah, Teti Estiasih, Yoshihito Iuchi, Harijono, Mamoru Yamada: Antioxidant activity of pigments derived from Monascus purpureus-fermented rice, corn, and sorghum, International Food Research Journal, 24: 1186-1191 (June 2017)

Matsutani M, Nantapong N, Murata R, Paisrisan P, Hirakawa H, Kataoka N, Yakushi T, Matsushita K. Complete genome sequencing of newly isolated thermotolerant Corynebacterium glutamicum N24 provides a new insights into its thermotolerant phenotype. J Biotechnol. 247: 29-33 (2017) 10 April 2017 doi: 10.1016/j.jbiotec.2017.02.025.

Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Production of 1,3-diols in Escherichia coli. Bioresour Technol. 245(Pt B):1538-154, 2017 Dec;doi:10.1016/j.biortech.2017.05.082.

Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Butyrate production under aerobic growth conditions by engineered Escherichia coli. J Biosci Bioeng. 123(5):562-568, 2017 May; doi: 10.1016/ j.jbiosc.2016.12.008.

Yoshida Y, Kuroiwa H, Shimada T, Yoshida M, Ohnuma M, Fujiwara T, Imoto Y, Yagisawa F, Nishida K, Hirooka S, Misumi O, Mogi Y, Akakabe Y, Matsushita K, Kuroiwa T. Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments. Proc Natl Acad Sci USA 114 (50): 13284-13289, 2017 Dec 12; doi: 10.1073/pnas.1715008114.

Yakushi T, Komatsu K, Matsutani M, Kataoka N, Vangnai AS, Toyama H, Adachi O, Matsushita K. Improved heterologous expression of the membrane-bound quinoprotein quinate dehydrogenase from Gluconobacter oxydans. Protein Expr Purif. 145:100-107, 2018 May; doi: 10.1016/j.pep.2018.01.007.

Yakushi T, Terada Y, Ozaki S, Kataoka N, Akakabe Y, Adachi O, Matsutani M, Matsushita K. Aldopentoses as new substrates for the membrane-bound, pyrroloquinoline quinone-dependent glycerol (polyol) dehydrogenase of Gluconobacter sp. Appl Microbiol Biotechnol. 102(7):3159-3171, 2018 Apr; doi: 10.1007/s00253- 018-8848-1. 

Matsumoto N, Hattori H, Matsutani M, Matayoshi C, Toyama H, Kataoka N, Yakushi T, Matsushita K. A single-nucleotide insertion in a drug transporter gene induces a thermotolerant phenotype  of Gluconobacter frateurii by increasing the NADPH/ NADP+ ratio via metabolic change.Appl Environ Microbiol. 2018 Mar 16. pii: AEM.00354-18. doi: 10.1128/AEM.00354-18.

Yakushi T, Fukunari S, Kodama T, Matsutani M, Nina S, Karaoke N, Theeragool G, and Matsushita K. Role of a membrane-bound aldehyde dehydrogenase complex AldFGH in acetic acid fermentation with Acetobacter pasteurianus SKU1108. Appl. Microbiol. Biotechnol. 2018 in press

Hoshida H, Kidera K, Takishita R, Fujioka N, Fukagawa T, Akada R. Enhanced production of extracellular inulinase by the yeast Kluyveromyces marxianus in xylose catabolic state. J. Biosci. Bioeng. (in press).

Ortiz-Merino RA, Varela JA, Coughlan AY, Hoshida H, da Silveira WB, Wilde C, Kuijpers NGA, Geertman J-M, Wolfe1 KH and Morrissey JP. Ploidy Variation in Kluyveromyces marxianus Separates Dairy and Non-dairy Isolates. Front. Genet., 9: 94 (2018)

 

(著書) 

Hoshida H and Akada R, High-temperature bioethanol fermentation by conventional and nonconventional yeasts, "Biotechnology of yeasts and filamentous fungi", A. Sibirny (edt.), Springer, pp. 39-62 (2017)

Hoshida H, Yarimizu T, Akada R, Selection of error-less synthetic genes in yeast, "Synthetic DNA", Hughes RA (Edt.), Springer, pp. 237-246 (2017)

 

 

2016年度

(研究論文)

Ano Y, Hours RA, Akakabe Y, Kataoka N, Yakushi T, Matsushita K, Adachi O. Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose. Biosci Biotechnol Biochem, 81: 411-418 (2017) doi: 10.1080/ 09168451.2016.1254535.

Hoshida H, Kondo M, Kobayashi T, Yarimizu T, Akada R, 5´-UTR introns enhance protein expression in the yeast Saccharomyces cerevisiae. Appl Mcrobiol Biotechnol 101: 241 (2017) doi: 10.1007/ s00253-016-7891-z.

Ignatius S, Zubaidah E, Estiasih T, Yamada M, Harijono H: Comparison of Monascus purpureus growth, pigment production and composition on different cereal substrates with solid state fermentation. Biocatalysis and Agricultural Biotechnology, 7:181-186 (2016) doi:10.1016/j.bcab.2016.05.011

Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Butyrate production under aerobic growth conditions by engineered Escherichia coli. J Biosci Bioeng, 123: 562-568 (2017) doi: 10.1016/ j.jbiosc.2016.12.008.

Matsushita K, Azuma Y, Kosaka T, Yakushi T, Hoshida H, Akada R, Yamada M. Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations. Biosci Biotechnol Biochem, 80: 655-668 (2016) doi: 10.1080/09168451.2015.1104235.

Matsutani M, Hirakawa H, Hiraoka E, Theeragool G, Yakushi T, Matsushita K. Complete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, Acetobacter pasteurianus SKU1108, Provide a New Insight into Thermotolerance. Microbes Environ, 31: 395-400 (2016) doi: 10.1264/jsme2.ME16023.

Nakamura M, Suzuki A, Yarimizu T, Hoshida H, Akada R, A PCR protocol for amplification of DNA from complex templates. Accounts of Biotechnology Research, 3: 28 (2016)

Nuanpeng S, Thanonkeo S, Yamada M, Thanonkeo P.: Ethanol production from sweet sorghum juice at high temperatures using a newly isolated thermotolerant yeast Saccharomyces cerevisiae DBKKI Y-53. Energies 9(4), 253 (2016); DOI 10.3390/en9040253

Sainz F, Jesús Torija M, Matsutani M, Kataoka N, Yakushi T, Matsushita K, Mas A. Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains. Front Microbiol, 7: 1358 (2016) doi: 10.3389/fmicb.2016.01358.

Suprayogi, Nurcholis M, Murata M, Lertwattanasakul N, Kosaka T, Rodrussamee N, Limtong S, Yamada M. Characteristics of kanMX4-inserted mutants that exhibit 2-deoxyglucose resistance in thermotolerant yeast Kluyveromyces marxianus. The Open Biotechnology Journal, 10: 208-222 (2016) doi: 10.2174/18740707016100100208

 

(著書)

Matsushita K and Matsutani M: Chapter 7. Distribution, evolution and physiology of oxidative fermentation, "Acetic acid bacteria: Ecology and Physiology", K. Matsushita et al. (eds.), Springer, pp. 159-178 (2016) ISBN 978-4-431-55931-3.

Adachi O & Yakushi T: Chapter 13. Membrane-bound dehydrogenases of acetic acid bacteria "Acetic acid bacteria: Ecology and Physiology", K. Matsushita et al. (eds.), Springer, pp. 273-297 (2016) ISBN 978-4-431-55931-3.

 

(その他)

村田正之,スカンヤ ニチヨン,チャンソム ケオオウドン,スプラヨギ,サビトリー リムトン,高坂 智之山田 守:第二世代バイオマス(セルロース系バイオマス)のエタノール変換・生産技術 化学工業,67: 519-528 (2016)

 

(特許)

・特許出願:中村美紀子,赤田倫治星田尚司;特願2015-220058「タグペプチド」

・特許出願:赤田倫治星田尚司,中村美紀子;特願2015-156575「ポリペプチド抽出方法」

・特許出願:中村美紀子,赤田倫治星田尚司;特願2015-007674「ターミネータ配列とプロモータ配列とを順次備えた線状二本鎖DNA」

・特許登録:山田 , 藤元奈保子;特許 5769187「セルロース原料の分解能を有する微生物、及びこれを用いたセルロース原料の分解処理」(登録日:平成27年7月3日)

・国際出願:山田 , スカンヤ ニチョン, ケオ-ウドネ チャンソム 国際出願番号:PCT/JP2015/002175「キシロース資化能及びエタノール生産能を有する酵母」(国際出願日:2015年4月22日)

 

2015年度

(研究論文)

Charoenyingcharoen P, Matsutani M, Yakushi T, Theeragool G, Yukphan P, Matsushita K. A functionally critical single nucleotide polymorphism in the gene encoding the membrane-bound alcohol dehydrogenase found in ethanol oxidation-deficient Gluconobacter thailandicus. Gene 567: 201-207 (2015) doi: 10.1016/j.gene.2015.04.080.

Charoensopharat K, Thanonkeo P, Thanonkeo S, Yamada M. Ethanol production from Jerusalem artichoke tubers at high temperature by newly isolated thermotolerant inulin-utilizing yeast Kluyveromyces marxianus using consolidated bioprocessing. Antonie Van Leeuwenhoek, 108: 173-190 (2015) doi: 10.1007/s10482-015-0476-5.

Kataoka N, Matsutani M, Yakushi T, Matsushita K. Efficient Production of 2,5-Diketo-d-Gluconate via Heterologous Expression of 2-Ketogluconate Dehydrogenase in Gluconobacter japonicus. Appl Environ Microbiol. 81: 3552-3560 (2015) doi: 10.1128/AEM.04176-14.

Kataoka N, Vangnai AS, Pongtharangkul T, Tajima T, Yakushi T, Matsushita K, Kato J. Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli. J Biotechnol. 204: 25-32 (2015) doi: 10.1016/j.jbiotec.2015.03.027.

Kawai M, Higashiura N, Hayasaki K, Okamoto N, Takami A, Hirakawa H, Matsushita K, Azuma Y. Complete genome and gene expression analyses of Asaia bogorensis reveal unique responses to culture with mammalian cells as a potential opportunistic human pathogen. DNA Res. 22: 357-366 (2015) doi: 10.1093/dnares/dsv018.

Keo-oudone C, Nitiyon S, Sotitham P, Tani A, Lertwattanasakul N, Yuangsaard N, Bounphanmy S, Limtong S, Yamada M. Isolation and characterization of thermotolerant ethanol-fermenting yeasts from Laos and application of whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) analysis for their quick identification. African Journal of Biotechnology, 15: 153-164 (2016) doi: 10.5897/AJB2015.14984.

Kitagawa T, Okita H, Baron B, Tokuda K, Nakamura M, Wang Y, Akada J, Hoshida H, Akada R, Kuramitsu Y, Nakamura K, Mutant screening for oncogenes of Ewing's sarcoma using yeast. Appl Microbiol Biotechnol, 99: 3737 (2015) doi: 10.1007/s00253-015-6621-2.

Matsutani M, Ito K, Azuma Y, Ogino H, Shirai M, Yakushi T, Matsushita K. Adaptive mutation related to cellulose producibility in Komagataeibacter medellinensis (Gluconacetobacter xylinus) NBRC 3288. Appl Microbiol Biotechnol. 99: 7229-7240 (2015) doi: 10.1007/s00253-015-6598-x.

Murata M, Nitiyon S, Lertwattanasakul N, Sootsuwan K, Kosaka T, Thanonkeo P, Limtong S, Yamada M. High-temperature Fermentation Technology for Low-cost Bioethanol. Journal of the Japan Institute of Energy, 94: 1154-1162 (2015) doi: 10.3775/jie.94.1154.

Nakamura M, Suzuki A, Akada J, Tomiyoshi K, Hoshida H, Akada R, End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter. Mol Biotechnol, 57: 1018 (2015) doi: 10.1007/s12033-015-9890-1.

Nakamura M, Suzuki A, Akada J, Yarimizu T, Iwakiri R, Hoshida HAkada R, A Novel Terminator Primer and Enhancer Reagents for Direct Expression of PCR-Amplified Genes in Mammalian Cells. Mol Biotechnol, 57: 767 (2015) doi: 10.1007/s12033-015-9870-5.

Nitiyon S, Keo-Oudone C, Murata M, Lertwattanasakul N, Limtong S, Kosaka T, Yamada M. Efficient conversion of xylose to ethanol by stress-tolerant Kluyveromyces marxianus BUNL-21. Springerplus, 5: 185 (2016) doi: 10.1186/s40064-016-1881-6.

Saichana N, Matsushita K, Adachi O, Frébort I, Frébortová J. Acetic acid bacteria: A group of bacteria with versatile biotechnological applications. Biotechnol Adv. 33, 1260-1271 (2015) doi: 10.1016/j.biotechadv. 2014.12.001.

Saichana N, Tanizawa K, Pechoušek J, Novák P, Yakushi T, Toyama H, Frébortová J. PqqE from Methylobacterium extorquens AM1: a radical S-adenosyl-l-methionine enzyme with an unusual tolerance to oxygen. J Biochem. 159: 87-99 (2016) doi: 10.1093/jb/mvv073.

Soemphol W, Tatsuno M, Okada T, Matsutani M, Kataoka N, Yakushi T, Matsushita K. A novel Na+(K+)/H+ antiporter plays an important role in the growth of Acetobacter tropicalis SKU1100 at high temperatures via regulation of cation and pH homeostasis. J Biotechnol. 211: 46-55 (2015) doi: 10.1016/ j.jbiotec.2015.06.397.

Suprayogi, Nguyen MT, Lertwattanasakul N, Rodrussamee N, Limtong S, Kosaka T, Yamada M. A Kluyveromyces marxianus 2-deoxyglucose-resistant mutant with enhanced activity of xylose utilization. Int Microbiol, 18: 235-244 (2015) doi: 10.2436/20.1501.01.255.

Suzuki A, Fujii H, Hoshida H, Akada R., Gene expression analysis using strains constructed by NHEJ-mediated one-step promoter cloning in the yeast Kluyveromyces marxianus. FEMS Yeast Res, 15, fov059 (2015) doi: 10.1093/femsyr/fov059.

Talukder AA, Sujon SI, Hossain MM, Gomes DJ, Yamada M. Production of Bioethanol at High Temperature from Tari. AiM, 05: 325-335 (2015) doi: 10.4236/aim.2015.55033.

Yamamoto H, Shima T, Yamaguchi M, Mochizuki Y, Hoshida H, Kakuta S, Kondo-Kakuta C, Noda NN, Inagaki F, Itoh T, Akada R, Ohsumi Y, The Thermotolerant Yeast Kluyveromyces marxianus Is a Useful Organism for Structural and Biochemical Studies of Autophagy. J Biol Chem, 290: 29506 (2015) doi: 10.1074/jbc.M115.684233.

Yarimizu T, Nakamura M, Hoshida H, Akada R, Screening of accurate clones for gene synthesis in yeast. J Biosci Bioeng, 119: 251 (2014) doi: 10.1016/j.jbiosc.2014.08.006.

 

(その他)

村田正之, 高坂智之, サビトリー リムトン, 山田 :省エネ型エタノール発酵新技術とセルロース系バイオマス変換基盤技術; 化学工業, 1月号: 1-6 (2015)

山田 , 赤田 倫治, 高坂 智之, 東 慶直, 星田 尚司, 松下 一信:耐熱性発酵微生物の耐熱性を付与する分子機構,化学と生物,53 (11): 763-773 (2015)

 

2014年度

(研究論文)

Fujioka Y, Suzuki SW, Yamamoto H, Kondo-Kakuta C, Kimura Y, Hirano H, Akada R, Inagaki F, Ohsumi Y, Noda NN, Structural basis of starvation-induced assembly of the autophagy initiation complex. Nat Struct Mol Biol, 21: 513 (2014) doi: 10.1038/nsmb.2822

Kataoka N, Vangnai AS, Ueda H, Tajima T, Nakashimada Y, Kato J. Enhancement of (R)-1,3-butanediol production by engineered Escherichia coli using a bioreactor system with strict regulation of overall oxygen transfer coefficient and pH. Biosci Biotechnol Biochem, 78 (4): 695-700 (2014)

Kawai S, Yakushi T, Matsushita K, Kitazumi Y, Shirai O, Kano K. Role of a non-ionic surfactant in direct electron transfer-type bioelectrocatalysis by fructose dehydrogenase. Electrochimica Acta 152, 19-24 (2015) doi:10.1016/j.electacta. 2014.11. 113

Kosaka T, Toh H, Fujiyama A, Sakaki Y, Watanabe K, Meng XY, Hanada S, Toyoda A. Physiological and genetic basis for self‐aggregation of a thermophilic hydrogenotrophic methanogen, Methanothermobacter strain CaT2. Environmental Microbiology Reports, 6: 268-277 (2014) doi: 10.1111/1758-2229.12128.

Lertwattanasakul N, Kosaka T, Hosoyama A, Suzuki Y, Rodrussamee N, Matsutani M, Murata M, Fujimoto N, Suprayogi, Tsuchikane K, Limtong S, Fujita N, Yamada M. Genetic basis of the highly efficient yeast Kluyveromyces marxianus: complete genome sequence and transcriptome analyses. Biotechnology for Biofuels, 8: 47 (2015) doi: 10.1186/s13068-015-0227-x.

Matsutani M, Fukushima K, Kayama C, Arimitsu M, Hirakawa H, Toyama H, Adachi O, Yakushi T, Matsushita K.  Replacement of a terminal cytochrome c oxidase by ubiquinol oxidase during the evolution of acetic acid bacteria. Biochim Biophys Acta. 1837: 1810-20 (2014) doi: 10.1016/j.bbabio.2014.05.355.

Matsutani M, Suzuki H, Yakushi T, Matsushita K. Draft genome sequence of Gluconobacter thailandicus NBRC 3257. Stand Genomic Sci. 9: 614-623 (2014) doi: 10.4056/sigs.4778605.

Nishikura-Imamura S, Matsutani M, Insomphun C, Vangnai AS, Toyama H, Yakushi T, Abe T, Adachi O, Matsushita K. Overexpression of a type II 3-dehydroquinate dehydratase enhances the biotransformation of quinate to 3-dehydroshikimate in Gluconobacter oxydans. Appl Microbiol Biotechnol. 98: 2955-2963 (2014) doi: 10.1007/s00253-013-5439-z.

Yarimizu T, Nakamura M, Hoshida HAkada R, Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus, Microbial Cell Fact, 14: 20 (2015) doi: 10.1186/s12934-015-0203-y.

 

(著書)

Nakamura M, Suzuki A, Hoshida H, Akada R, Minimum GC-rich sequences for overlap extension PCR and primer annealing, "Methods Mol Biol series: DNA Cloning and Assembly Methods", Valla S and Lale R (eds.), Springer, pp. 165-181 (2014) ISBN: 978-1-62703-763-1.

Hoshida H, Yarimizu T, Akada R, Selection of Error-Less Synthetic Genes in Yeast. "Methods Mol Biol series: Synthetic DNA Cloning", Hughes RA (eds.), Springer, pp. 237-246 (2014) ISBN: 978-1-4939-6341-6

 

(特許)

 ・山田 , スカンヤ ニチョン;特開2015-73508「キシロース資化能を有するエタノール生産酵母を生産する方法」(出願日:2014年4月28日)

星田尚司赤田倫治:特願2014-158646「高発現プロモーター」国立大学法人山口大学,平成26年8月4日

山田 , スカンヤ ニチョン, ケオ-ウドネ チャンソム:特願2014-092206「キシロース資化能及びエタノール生産能を有する酵母」山口大学(2014年4月28日出願)

 ・赤田倫治星田尚司山田 ,村田正之,高坂智之松下一信薬師寿治,ナンタポン・ナワラート,東慶直:特願2014-159048「耐熱性微生物の作製方法」山口大学・近畿大学(2014年8月4日出願)