2024.04.18
The luciferase-based in vivo protein-protein interaction assay revealed that CHK1 promotes PP2A and PME-1 interaction
A research group led by Associate Professor Tsuyoshi Ohama of Yamaguchi University, in collaboration with RIKEN, has elucidated a novel DNA repair mechanism activated when cancer cells sustain DNA damage. This discovery was achieved by applying a protein-protein interaction analysis technique utilizing the bioluminescent enzyme from deep-sea shrimp.
The study identified the role of the molecule CHK1 in controlling the binding between the tumor suppressor PP2A and PME-1, which inhibits PP2A’s function. It revealed that CHK1 phosphorylates PP2A, strengthening their binding, and that PME-1 prevents the inactivation of CHK1, which is necessary for rapid DNA repair.
This research paper was published in the Journal of Biological Chemistry.
- Division of Translational Research for One Medicine
- Takashi Ohama（Basic Veterinary Science）
- Paper Title：The luciferase-based in vivo protein-protein interaction assay revealed that CHK1 promotes PP2A and PME-1 interaction
- Authors：Sana Ando, Keiko Tanaka, Maharu Matsumoto, Yuki Oyama, Yuri Tomabechi, Atsushi Yamagata, Mikako Shirouzu, Reiko Nakagawa, Noriaki Okimoto, Makoto Taiji, Koichi Sato, Takashi Ohama
- Publication：Journal of Biological Chemistry
- Posted: April 6, 2024 (Online Release)
- DOI：https://doi.org/10.1016/j.jbc.2024.107277
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