Achievements


2010”N“x

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¡ Ano Y, Shinagawa E, Adachi O, Toyama H, Matsushita K.
Selective and high conversion of D-glucose to 5-keto-D-gluoconate by Gluconobacter suboxydans.
Biosci Biotechnol Biochem. 2011;75(3):586-9. doi: 10.1271/bbb.100701. Epub 2011 Mar 7.

¡ Matsutani M, Hirakawa H, Yakushi T, Matsushita K.
Genome-wide phylogenetic analysis of Gluconobacter, Acetobacter, and Gluconacetobacter.
FEMS Microbiol Lett. 2011 Feb;315(2):122-8.

¡ de Mendonca JD, Adachi O, Rosado LA, Ducati RG, Santos DS, Basso LA.
Kinetic mechanism determination and analysis of metal requirement of dehydroquinate synthase from Mycobacterium tuberculosis H37Rv: an essential step in the function-based rational design of anti-TB drugs.
Mol Biosyst. 2011 Jan;7(1):119-28.

¡ Adachi O, Hours RA, Akakabe Y, Tanasupawat S, Yukphan P, Shinagawa E, Yakushi T, Matsushita K.
Production of 4-keto-D-arabonate by oxidative fermentation with newly isolated Gluconacetobacter liquefaciens.
Biosci Biotechnol Biochem. 2010;74(12):2555-8. doi: 10.1271/bbb.100698. Epub 2010 Dec 7.

¡ Adachi O, Ano Y, Shinagawa E, Yakushi T, Matsushita K.
Conversion of quinate to 3- dehydroshikimate by Ca-alginate-immobilized membrane of Gluconobacter oxydans IFO 3244 and subsequent asymmetric reduction of 3-dehydroshikimate to shikimate by immobilized cytoplasmic NADP-shikimate dehydrogenase.
Biosci Biotechnol Biochem. 2010;74(12):2438-44. doi: 10.1271/bbb.100497. Epub 2010 Dec 7.

¡ Kubota K, Miyazono K, Nagata K, Toyama H, Matsushita K, Tanokura M.
Crystallization and preliminary X-ray analysis of 5-keto-D-gluconate reductase from Gluconobacter suboxydans IFO12528 complexed with 5-keto-D-gluconate and NADPH.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1680-2.

¡ Habe H, Shimada Y, Fukuoka T, Kitamoto D, Itagaki M, Watanabe K, Yanagishita H, Yakushi T, Matsushita K, Sakaki K.
Use of a Gluconobacter frateurii mutant to prevent dihydroxyacetone accumulation during glyceric acid production from glycerol.
Biosci Biotechnol Biochem. 2010;74(11):2330-2. doi: 10.1271/bbb.100406. Epub 2010 Nov 7.

¡ Kanchanarach W, Theeragool G, Inoue T, Yakushi T, Adachi O, Matsushita K.
Acetic acid fermentation of Acetobacter pasteurianus: Relationship between acetic acid resistance and pellicle polysaccharide formation.
Biosci Biotechnol Biochem. 2010;74(8):1591-7. doi: 10.1271/bbb.100183. Epub 2010 Aug 7.

¡ Yoshida Y, Kuroiwa H, Misumi O, Yoshida M, Ohnuma M, Fujiwara T, Yagisawa F, Hirooka S, Imoto Y, Matsushita K, Kawano S, Kuroiwa T.
Chloroplasts divide by contraction of a bundle of nanofilaments consisting of polyglucan.
Science. 2010 Aug 20;329(5994):949-53.

¡ Habe H, Fukuoka T, Morita T, Kitamoto D, Yakushi T, Matsushita K, Sakaki K.
Disruption of the membrane-bound alcohol dehydrogenase-encoding gene improved glycerol use and dihydroxyacetone productivity in Gluconobacter oxydans.
Biosci Biotechnol Biochem. 2010;74(7):1391-5. doi: 10.1271/bbb.100068. Epub 2010 Jul 7.

¡ Shinagawa E, Adachi O, Ano Y, Yakushi T, , Matsushita K.
Purification and characterization of membrane-bound 3-dehydroshikimate dehydrogenase from Gluconobacter oxydans IFO3244, a new enzyme catalyzing extracellular protocatechuate formation.
Biosci Biotechnol Biochem. 2010;74(5):1084-8. doi: 10.1271/bbb.100043. Epub 2010 May 7.

¡ A. S. Vangnai, W. Promden, W. De Eknamkul, K. Matsushita, H. Toyama.
Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244.
Biochemistry (Moscow), 2010;75(4):452-9.

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Ÿ Yakushi T, and Matsushita K.
Alcohol dehydrogenase of acetic acid bacteria, mode of action and applications in biotechnology.
App. Microbiol. Biotechnol. 86 (5) 1257-1265 (2010)



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